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Ativan 1 mg effet secondaire (15 mg/kg) in water was administered to rats at doses of 0, 0.5, 1, 7, and 10 mg/kg bw/day for 8 days. Toxicity of the compound was determined based on the histopathological study and oral absorption measurements. Histopathological examination of the bladder,
Best online pharmacy new zealand kidneys and lymph nodes revealed mild to moderate nephropathy. As compared 2 mg/kg bw/day, there was a decrease in the amount of glomerular filtration rate and also decreased renal tubular epithelial thickness in rats treated with 5 mg/kg bw/day and up to 7 mg/kg bw/day. Oral absorption of 0.5 and 1 mg/kg bw/day were shown to be negligible.
(D) Dose-response experiments of compound A at dose 10 mg/kg bw/day in C57/BL6 mice was carried out during a 4-week period. Toxicity of the compound was determined based on the histopathological study and oral absorption measurements.
(E) The mechanism of action compound A is evaluated using microdialysis on human bronchial epithelial cells.
(F) Effect of the compound on liver function.
EXAMPLE 5
Test
The following test is made to identify compounds with similar or effective mechanisms of action to the present invention. In manner, three compounds with similar or effective mechanisms of action to the present invention can be tested on three different cell types using the same test procedure in parallel.
Example 1
Toxicity of aqueous extract L. rhamnosus CNCM I-11 on the microsomal enzyme activity evaluated by the method known as in vitro microsomal enzyme activity assay was carried out in order to understand the vitro activity of present invention. For the results of assay one compound with a molecular weight of 500 Da, having the chemical formula:
C20H25O2 is aqueous extract of L. rhamnosus CNCM I-11 having a molecular weight of 800 kDa. The amount drug extracted was 10 g. The reaction mixture was subjected to an anode at room temperature for 4 h using 25 mmol/L of sodium hypochlorite solution. In vitro microsomal enzyme activity was determined using a microbead microelectrode system (Waters Mini-Tek Biosystems, Tokyo, Japan).
The results of above-described testing showed that the reaction mixture containing 10 g of L. rhamnosus CNCM I-11 extract showed no toxicity of the extract on enzyme activity of microsomes as demonstrated by the reduction of activity as shown in the following graph:
Treatment of the extract with 10 g/L of sodium hypochlorite solution, which is an inhibitor in the microsomal enzyme activity, resulted in a significant decrease of activity as shown in the following graph:
Prevention of microsomal enzyme activity the extract had a significantly beneficial effect on the in vitro toxicity of extract on microsomal enzyme activity as shown in the following graphic-Figure 1:
Example 2
Toxicity of aqueous extract L. rhamnosus CNCM I-11 on the enzyme
cost of dexamethasone tablets uk activity evaluated by method known as dexamethasone cost australia in vitro enzyme activity assay was carried out in order to understand the vitro activity of present invention. For the results of assay one compound with a molecular weight of 200 Da, having the chemical formula:
C20H20O2 is aqueous extract of L. rhamnosus CNCM I-11 having a molecular weight of 800 kDa. The amount drug extracted was 10 g. The reaction mixture was subjected to aqueous and neutral pH conditions in the presence of 10 mmol/L, 5 mmol/L and 2 respectively, of sodium phosphate triphosphate. In vitro enzyme activity was determined using microbead microelectrode system (Waters Mini-Tek Biosystems).
Prevention of microsomal enzyme activity the extract had a significantly beneficial effect on the in vitro toxicity of extract on microsomal
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Example 3
Toxicity of aqueous extract L. rhamnosus CNCM I-11 on the enzyme activity evaluated by method known as in vitro enzyme activity assay was carried out in order to understand the vitro activity of present invention.
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